Tag Archive for Enterocytozoon bieneusi

Chapter 8 – Characterization of Genotypes of Enterocytozoon bieneusi in Immunosuppressed and Immunocompetent Patient Groups.

Abstract

A retrospective phylogenetic analysis was performed on isolates of Enterocytozoon bieneusi to characterize the genotypes in different patient cohorts. Fifty-seven isolates, collected from patients living in Malawi and The Netherlands, were classified by age and immune status of the hosts. Sequence analysis of the internal transcribed spacer (ITS) region identified 16 genotypes; nine have not previously been described. Genotypes K and D were most prevalent among patient groups, whereas genotype C was restricted to transplantation patients receiving immunosupressives and genotype B showed a predisposition towards patients living with HIV/AIDS. Different genotypes showed more dispersion among isolates from Malawi compared with those from The Netherlands. A constructed map estimating the genealogy of the ITS region reveals a dynamic evolutionary process between the genotypes.

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Robert J. ten Hove, Lisette van Lieshout, Mike B.J. Beadsworth, M. Arantza Perez, Klaartje Spee, Eric C.J. Claas, Jaco J. Verweij. The Journal of Eukaryotic Microbiology (2009)

Chapter 7 – Multiplex Detection of Enterocytozoon bieneusi and Encephalitozoon spp. in Fecal Samples using Real-time PCR.

Abstract

A multiplex real-time polymerase chain reaction (PCR) method was developed for the simultaneous detection of Enterocytozoon bieneusi (n = 30) and Encephalitozoon spp. (n = 3) in stool samples. The multiplex PCR also included an internal control to detect inhibition of the amplification by fecal constituents in the sample. The assay was performed on species-specific DNA controls (n = 22) and a range of well-defined stool samples (n = 140), and it achieved 100% specificity and sensitivity. The use of this assay in a diagnostic laboratory offers the possibility of introducing DNA detection as a feasible technique in the routine diagnosis of intestinal microsporidian infections.

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Jaco J. Verweij, Robert ten Hove, Eric A.T. Brienen, Lisette van Lieshout. Diagnostic Microbiology and Infectious Disease (2007), 57 (2): 163-167